Genetic Polymorphism Under Therapy Genotypes From Cucumber (Cucumis sativus) And Sunflower (Helianthus annuus)
DOI:
https://doi.org/10.23918/eajse.v10i3p5Keywords:
DNA, Denaturation, Genetic Polymorphism, Genotype, Hybrid, HeterogeneityAbstract
Seven F1 hybrids of Sunflower and Cucumber from two families were studied, both lines and hybrids of the first generation. The denaturations processes of kinetics of different genotypes isolated from DNA of Sunflower and Cucumber was investigated. The thermal denaturation of DNA macromolecules have proved high melting temperatures at sunflower hybrids, when compared with cucumber genotypes. This fact denotes the increased stability of DNA macromolecules. The level of heterogeneity has varied in dependence of genotype and it has correlated with the highest values of morphological and physiological parameters which determines the effect of hybrid vigor in the studies of Sunflower and Cucumber genotypes. The kinetics of denaturation processes, realized from two cucumber families, has proved that the first generation hybrids DNA macromolecules have higher melting temperature , with an increased content of G-C content (%) and also a higher level of heterogeneity (2σ%) from Sunflower, in comparison with parental lines. Molecular studies on Sunflower and Cucumber would be a stepping stone and of great advantage to agriculture, where farmers deliberately crossbreed different strains or varieties of plants to take advantage of heterosis, thereby producing hybrids with improved traits such as; high crop yields, a better disease resistance or enhanced agricultural productivity.
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[1] Фадеева Т.С., Соснихина СП., Черкаева Н.М. Сравнительная генетика растений. - Ленинград, 1980. - 248 с.
[2] Raicu P. Genetica. Vol. II. - Bucureşti, 1997. - 238 p.
[3] Pierce B.A., Mitton I.B. The relationship between genome size and genetic variation // Am. Nat. - 1980. - P.850-861.
[4] Лобов В.П., Даскалюк А.П. Сравнительное исследование ДНК озимых и яровых форм пшеницы // Доклады АН СССР. - 1984. - Том 275. - №1. - С.218-221.
[5] Даскалюк А.П., Остаплюк А.Н., Тищенко Е.Н., Лобов В.П. Свойства уникальных и повторяющихся последова-тельностей ДНК родственных яровых и озимых сортов пшеницы // Физиология и биохимия культурных рас-тений. - 1982. - Том 14. - №2. - С.134-138.
[6] Даскалюк А.П., Остаплюк А.Н., Тищенко Е.Н., Порох Л.С., Лобов В.П. Структура генома гибрида кукурузы Буковинский 15 и его родительских форм. - В кн: Нуклеиновые кислоты и хроматин растений. - Киев: Наукова Думка, - 1981. - С. 57-61.
[7] Даскалюк А.П., Остаплюк А.Н., Тищенко Е.Н., Порох Л.С., Лобов В.П. Характеристика генома родственных сортов пшеницы. – В кн: Нуклеиновые кислоты и хроматин растений. - Киев: Наукова Думка, 1981, c.53-57.
[8] Боброва В.К. О степени сродства нуклеотидных последовательностей ДНК хлоропластов растений. – В кн: Нуклеиновые кислоты и хроматин растений. - Киев: Наукова Думка, 1981, c.14-18.
[9] Larson A. A reevaluation of the relationship between genome size and genetic variation // Am. Nat. - 1981. - P.119-125.
[10] Santa Lucia J., Hatim T., Seneviratne P. Improved Nearest-Neighbor parameters for Predicting DNA duplex stabi-lity // Biochemistry. - 1996. - Vol. 35. - P.3555-3562.
[11] Ussery D. W. DNA denaturation // Academic Press. - 2000. - 314 p.
[12] Аиала Ф.Д. Современная генетика. Тoм 2. - Москва: Мир, 1988. - 265 c.
[13] Айала Ф.Д. Современная генетика. Том 1. - Москва: Мир, 1987. - 280 с.
[14] Britten R.I., Graham O.B. Analysis repeating DNA sequences by reassociation // Meth. Enzimol. E. - 1974. - Vol.29. - P.363-418.
[15] Murray M.G., Thompson W.F Rapid isolation of molecular weight DNA // Nucleic Acids Research. - 1980. - Vol.8. - No.19. - P.4321-4325.
[16] Nover N. Melting computing the melting temperature of nucleic acid duplex // Applications note. - 2001. - Vol.17. - P.126-127.
[17] Orlandini E., Bhattachacharjee S., Maritan A. Mechanical denaturation of DNA: existence of a low-temperature denaturation // J. Physics A: Matematica land General. - 2001. - Vol.34. - P.751-756.
[18] Мaндель М., Мармур Дж. Определение содержания гуанина и цитозина в ДНК с помощью кривых плавления. – В кн: Методы исследования нуклеиновых кислот, 1986, c.182-192.
[19] Скрипка Л.В. Гетерогенность ДНК кукурузы. – В кн: Нуклеиновые кислоты и хроматин растений. - Киев: Наукова Думка, 1981, c.130-133.
[20] Maksim, SM. & Kirill, V. A. & Vera, A.G. Mitogenomic research of silverleaf sunflower (Helianthus argophyllus) and its interspecific hybrids. Current issues in Molecular Biology, 2023, 45(6):4861-4849. https://doi.org/10.3390/cimb45060308
[21] Zharaa, A.N. & Bahir, A. & Jawaad, K.A. Detection of genetic polymorphisms using random amplified polymorphic DNA (RAPD)-PCR Fenugreek (Trigonella foenum -graecum) Plants after seed treatment with biotic and abiotic agents. Journal of pure applied microbiology, 2021, 15(3):1409-1420. https://doi.org/10.22207/JPAM.15.3.33
[22] Tapan, K.M & Awdhesh, K.M. & Yugal, K.M & Ahmed, A. Space breeding: the next-generation crops. Frontiers in plant science, 2021, 12, 2407.
[23] Muayad, M.I. & Zeyad, A.A. Efficiency of selection in inducing genetic-molecular variations in sunflower. IOP Conference Series: Earth and Environmental Science, 2023, 1158(6), 062032
[24] Havya, A. & Yalcin, K. The genetic characterization of wild sunflower species (Helianthus species) and interspecific hybrids based on broomrape resistance. Agbiol Proceedings, 2021,1010.
[25] Zhang, J. & Yang, J.& Zhang, L. A new SNP Genotyping technology target SNP-seq and its application in genetic analysis of cucumber varieties. Sci rep, 2020, 10, 5623. https://doi.org/10.1038/s41598-020-62518-6.
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