First Derivative Spectrophotometric Determination of Cholesterol and Triglyceride in Human Serum

Abstract

Cholesterol is present in tissues and plasma lipoproteins either as free cholesterol or combined with a long chain fatty acid as cholesterol ester. Triglycerides are the simplest lipids constructed from fatty acids and glycerol. Derivative spectrophotometric techniques were used for determination of individual and simultaneous determination of cholesterol and triglyceride. First derivative spectrophotometric technique was used for determination of cholesterol and triglyceride in serum samples in the concentration ranges (10 – 100 μg/ml) for cholesterol with correlation coefficient 0.9983 and detection limit 2.9 μg/ml with good precision and accuracy basing upon measuring height at 218 nm depending upon the values of the relative standard deviation percentage (RSD %) and the relative error percentage (Error %) for ten replicate measurements of three different concentration and (15 – 150 μg/ml) for triglyceride with correlation coefficient 0.9972 and detection limit 5.5 μg/ml with good precision and accuracy basing upon measuring peak – to – baseline at 242 nm depending upon the values of the relative standard deviation percentage (RSD %) and the relative error percentage (Error %) for ten replicate measurements of three different concentration.