Derivative Spectrometric Determination of Glucose and Asparagine in Potato Samples
Keywords:
Derivative Spectrometric, Determination, Glucose, Asparagines, Potato SamplesAbstract
Glucose can be detected using the first derivative spectrum depending upon the multipacks at the range 290-350 nm, while it can be determined in the concentration range (3.0-9.0) at maximum positive absorption at 260 and 320. The second derivative spectrum of the glucose can be used for the determination of glucose in the concentration range (1.0-9.0) ppm at positive peak 240 nm and in the range (2.0-9.0) at negative peak. The first derivative spectrum of asparagine which can be used for the determination of asparagine in the concentration range (4.0-20) at positive peak 245 nm and at negative peak 290 nm respectively. Asparagine can be determined using the second derivative spectrum as shown in the concentration range (4.0-14) at positive peak 230 nm and at negative peak 270 nm respectively. The method has been applied for determination of glucose and asparagines in potato samples and the results were
compared with that obtained by HPLC.
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