Isolation, Biochemical Identification and Antioxidant Activity of Locally Isolated Lactobacillus spp in Garmian Area
DOI:
https://doi.org/10.23918/eajse.v3i1sip128Keywords:
Lactobacillus spp., Biochemical Tests, API 50 CHL, Antioxidant ActivityAbstract
The study was conducted in the Biology research laboratory In Garmian University during the period of summer to autumn 2016, which aimed to the isolation and biochemically identification of Lactobacillus sp. and comparing their antioxidant activity with Lactobacillus reuteri ATCC 23272, as well as both of the isolates and reference strain with ascorbic acid which used as a control. Out of 25 samples were collected from different sources such as vaginal swabs, infant stool, sheep and cow’s milk, six strains of Lactobacillus sp were Identified according to their colony morphology on MRS agar along with microscopic examination. Further identification strategies are carried out by biochemical analysis including catalase, oxidase, and API 50 CHL kit system through which strains as L. pentosus, L.acidophilus, L. collinoides, L. fermentum, L. salivarius and L. brevis were identified. The results of antioxidant activity which carried out by 2,2-DiPhenyl-2-Picryl hydrazyl hydrate ( DPPH) assay for all six isolated strains revealed that the best antioxidant activity was recorded by L. pentosus strain IC50=0.0464 which showed no-significantly differences with the ascorbic acid antioxidant activity IC50 = 0.0186, on the other hand, Lactobacillus reuteri ATCC 23272 exhibited antioxidant activity about IC50=1.998, in addition, other isolated strains L. acidophilus, L. fermentum, L. salivarius, L. collinoides and L. brevis demonstrates good antioxidant activity with IC50= 0.2311, 0.2792, 0.3707, 3.744 and 5.766 respectively.
References
Abubakr, M.A.S.; Hassan, Z.; Imdakim, M.M.A., & Sharifah, N. R. S. A. (2012). Antioxidant
activity of lactic acid bacteria (LAB) fermented skim milk as determined by 1, 1-diphenyl2-picrylhydrazyl (DPPH) and ferrous chelating activity (FCA). African Journal of
Microbiology Research, 6(34), 6358-6364. doi: 10.5897/AJMR12.702
Alvarez-Olmos, M.I., Barousse, M.M., Rajan, L., Van Der Pol, B.J., Fortenberry, D., Orr, D., &
Fidel Jr, P.L., (2004). Vaginal lactobacilli in adolescents: presence and relationship to local
and systemic immunity, and to bacterial vaginosis. Sexually Transmitted Diseases, 31(7),
393-400.
Arici, M., Bilgin, B., Sagdic, O., & Ozdemir, C. (2004). Some characteristics of Lactobacillus
isolates from infant feces. Food Microbiology, 21(1), 19-24. https://doi.org/10.1016/S0740-
0020(03)00044-3
Boyd, M.A., Antonio, M.A., & Hillier, S.L. (2005). Comparison of API 50 CH strips to wholechromosomal DNA probes for identification of Lactobacillus species. Journal of Clinical
Microbiology, 43(10), 5309-5311. doi: 10.1128/JCM.43.10.5309-5311.2005
Davoodabadi, A., Dallal, M.M.S., Lashani, E., & Ebrahimi, M.T. (2015). Antimicrobial Activity of
Lactobacillus spp. Isolated From Fecal Flora of Healthy Breast-Fed Infants Against
Diarrheagenic Escherichia coli. Jundishapur Journal of Microbiology, 8(12). doi:
10.5812/jjm.27852
Hassan,P., & K.K Peh. (2014). Isolation and identification of Lactobacillus
acidophilus. International Journal of Pharmacy and Pharmaceutical Sciences, 6,1.
Hoque, M.Z., Akter, F., Hossain, K.M., Rahman, M.S.M., Billah, M.M., & Islam, K.M.D. (2010).
Isolation, identification and analysis of probiotic properties of Lactobacillus spp. from
selective regional yoghurts. World Journal of Dairy & Food Sciences, 5(1), 39-46.
Klein, G., Zill, E., Schindler, R., & Louwers, J. (1998). Peritonitis Associated with VancomycinResistantLactobacillus rhamnosus in a Continuous Ambulatory Peritoneal Dialysis Patient:
Organism Identification, Antibiotic Therapy, and Case Report. Journal of Clinical
Microbiology, 36(6), 1781-1783.
Mahon, C.R., Lehman, D.C., & Manuselis Jr, G. (2014). Textbook of diagnostic microbiology.
Elsevier Health Sciences.
Nelson, G.M., & George, S.E. (1995). Comparison of media for selection and enumeration of mouse
fecal flora populations. Journal of Microbiological Methods, 22(3), 293-300.
https://doi.org/10.1016/0167-7012(95)00004-5
Ngongang, E.F.T., Tiencheu, B., Achidi, A.U., Fossi, B.T., Shiynyuy, D.M., Womeni, H.M., &
François, Z.N. (2016). Effects of Probiotic Bacteria from Yogurt on Enzyme and Serum
Cholesterol Levels of Experimentally Induced Hyperlipidemic Wistar Albino
Rats. American Journal of Biology and Life Sciences, 4(6), 48.
Ngongang, E.F.T., Tiencheu, B., Fossi, B.T., Achidi, A.U., Shiynyuy, D.M., Womeni, H.M., &
François, Z.N. (2016). Isolation and Identification of Cholesterol Lowering Probiotic
Bacteria from Palm Wine (Raffia mambillensis). Journal of Microbiology Research, 6(5),
93-102. doi:10.5923/j.microbiology.20160605.01
Nigatu, A. (2000). Evaluation of numerical analyses of RAPD and API 50 CH patterns to
differentiate Lactobacillus plantarum, Lact. fermentum, Lact. rhamnosus, Lact. sake, Lact.
parabuchneri, Lact. gallinarum, Lact. casei, Weissella minor and related taxa isolated from
kocho and tef. Journal of Applied Microbiology, 89(6), 969-978. doi: 10.1046/j.1365-
2672.2000.01202.x
Nyanzi, R., Shuping, D.S., Jooste, P.J., & Eloff, J.N. (2015). Antibacterial and Antioxidant Activity
of Extracts from Selected Probiotic Bacteria. Journal of Food Research, 4(5),122.
https://dx.doi.org/10.5539/jfr.v4n5p122
Ogunshe, A.A. (2008). Bioinhibition of diarrhogenic Gram-positive bacterial pathogens by potential
indigenous probiotics. Asian Pacific Journal of Tropical Medicine, 1(2), 7-11.
Ozgun, D., & Vural, H.C. (2011). Identification of Lactobacillus strains isolated from faecal
specimens of babies and human milk colostrum by API 50 CHL system. Journal of Medical
Genetics and Genomics, 3(3), 46-49.
Pascual, L.M., Daniele, M.B., Pájaro, C., & Barberis, L. (2006). Lactobacillus species isolated from
the vagina: identification, hydrogen peroxide production and nonoxynol-9
resistance. Contraception, 73(1), 78-81. https://doi.org/10.1016/j.contraception.2005.06.066
Risan, M.H., Abdulmohimin, N., & Mohammad-Amin, S.M. (2016). Production, Partial Purification
and Antitumor Properties of Bioactive Compounds from Locally Isolated Actinomycetes
(KH14). Iraqi Journal of Biotechnology, 15(3), 51-64.
Sneath, P.H.S.; Mair, N.S.; Sharpe, M. E., & Holt, J. C. (2009). Bergey’s Manual of Systematic
Bacteriology. Baltimore, Williams and Wikins.
Valko, K., Du, C., Bevan, C., Reynolds, D.P., & Abraham, M.H. (2001). Rapid method for the
estimation of octanol/water partition coefficient (log Poct) from gradient RP-HPLC
retention and a hydrogen bond acidity term (Sigma alpha2H). Current Medicinal
Chemistry, 8(9), 1137-1146. https://doi.org/10.2174/0929867013372643
Vardapetyan, H.R., Blbulyan, S.S., Tiratsuyan, S.G., & Trchounian, A.H. (2012). Elucidation of
Antiradical and Antibacterial Properties of Hypericum Perforatum Extracts. Հ Հ ԳԱԱ
Զ ե կ ո ւ յ ց ն ե ր , 112(3), 282-290.
Venkatachalam, R.N., Singh, K., & Marar, T. (2012). Phytochemical screening in vitro antioxidant
activity of Psidium guajava. Free Radicals and Antioxidants, 2(1), 31-36.
https://doi.org/10.5530/ax.2012.2.7
Wan, Y., Xin, Y., Zhang, C., Wu, D., Ding, D., Tang, L., Owusu, L., Bai, J., & Li, W. (2014).
Fermentation supernatants of Lactobacillus delbrueckii inhibit growth of human colon
cancer cells and induce apoptosis through a caspase 3-dependent pathway. Oncology
letters, 7(5), 1738-1742. https://doi.org/10.3892/ol.2014.1959
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Eurasian J. Sci. Eng is distributed under the terms of the Creative Commons Attribution License 4.0 (CC BY-4.0) https://creativecommons.org/licenses/by/4.0/
