Metallic and Chemical Composition of Wild Plant (Rheum Ribes): A Natural Superfood with Hidden Elements
DOI:
https://doi.org/10.23918/eajse.v11i2p10Keywords:
Rheum Ribes, Metal Analysis, Crude Protein, Total Ash, Moisture Content, Bioactive Constituents, Therapeutic EffectsAbstract
The natural product Rheum ribes, also known as Rivas in the Kurdistan area, is considered one of the most nutritious wild rhubarbs and is valued both as a dietary and medicinal resource. It is commonly referred to as R. ribes in several scientific databases, due to the presence of anthracene derivatives-compounds known for their laxative and anti-inflammatory properties R. ribes is recognised as a therapeutic herb traditionally used in medicine. This study highlights its potential as a natural superfood rich in bioactive constituents and trace elements beneficial to human health. The metal content analysis identified essential elements including Iron, Zinc, manganese, potassium and copper with concentrations of 145.755, 34.437, 3364.568, 28577.680 and 29.038 μg/mL, respectively, and limit of detection (LOD) at 0.0013, 0.0006, 0.0005, 0.0022 and 0.0048 μg/mL, respectively. Trace metals were quantified using a sophisticated analytical method, Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) technique. Average chemical composition of R. ribes: total ash (9.542%), moisture content (7.655%), nitrogen content (4.26%), crude protein (26.62%), and pH (7.4).
References
[1] Keshavarzi Z, Shakeri F, Maghool F, Jamialahmadi T, Johnston TP, Sahebkar A. A review on the phytochemistry, pharmacology, and therapeutic effects of Rheum ribes. Natural Products and Human Diseases: Pharmacology, Molecular Targets, and Therapeutic Benefits. 2021:447-61. https://doi.org/10.1007/978-3-030-73234-9_30.
[2] HS Hussain F, Majedi S, Abdulsattar Faraj T, Ozdemir M, Ahamad J, H KARIM TA, Dilawer Issa K, Q Mustafa M. Documentation of Ethnomedicinal Uses of Wild Plants Growing in Kodo Mountain by Kurdish Tribe of Iraq. Journal of the Chemical Society of Pakistan. 2024;46(4). https://doi.org/10.52568/001432/JCSP/46.02.2024.
[3] Andiç S, Tunçtürk Y, Ocak E, Köse S. Some chemical characteristics of edible wild Rhubarb species (Rheum ribes L.). Res J Agric Biol Sci. 2009;5(6):973-7. https://www.researchgate.net/publication/242629972.
[4] Ghorbani A, Amiri MS, Hosseini A. Pharmacological properties of Rheum turkestanicum Janisch. Heliyon. 2019 Jun 1;5(6). https://doi.org/10.1016/j.heliyon.2019.e01986.
[5] Ahmed HM. Ethnopharmacology of medicinal plants used in choman community, Hewler (Erbil), Kurdistan Region, Iraq. Genetic Resources and Crop Evolution. 2024 Aug;71(6):2797-817. https://doi.org/10.1007/s10722-023-01800-8.
[6] Blancquaert D, De Steur H, Gellynck X, Van Der Straeten D. Metabolic engineering of micronutrients in crop plants. Annals of the New York Academy of Sciences. 2017 Feb;1390(1):59-73. https://doi.org/10.1111/nyas.13274.
[7] Andrews P, Johnson RJ. Evolutionary basis for the human diet: consequences for human health. Journal of internal medicine. 2020 Mar;287(3):226-37. https://doi.org/10.1111/joim.13011.
[8] Dias DA, Urban S, Roessner U. A historical overview of natural products in drug discovery. Metabolites. 2012 Apr 16;2(2):303-36. https://doi:10.3390/metabo2020303.
[9] Uyar P, Coruh N, İscan M. Evaluation of in vitro antioxidative, cytotoxic and apoptotic activities of Rheum ribes ethyl acetate extracts. Journal of Plant Sciences. 2014;2(6):339-46. https://doi: 10.11648/j.jps.20140206.22.
[10] Harvey AL. Natural products in drug discovery. Drug discovery today. 2008 Oct 1;13(19-20):894-901. https://doi:10.1016/j.drudis.2008.07.004.
[11] Gomes C, Rautureau M. Minerals latu sensu and human health. Benefits, Toxicity and Pathologies. Cham: Springer. 2021. https://doi.org/10.1007/978-3-030-65706-2.
[12] Tuncturk M, Celen AE, Tuncturk R. NUTRIENT CONTENT OF THREE EDIBLE WILD PLANTS FROM POLYGONACEAE FAMILY. Oxidation Communications. 2017 Jan 15;40. https://www.researchgate.net/publication/316958678.
[13] Godswill AG, Somtochukwu IV, Ikechukwu AO, Kate EC. Health benefits of micronutrients (vitamins and minerals) and their associated deficiency diseases: A systematic review. International Journal of Food Sciences. 2020 Jan 7;3(1):1-32. https://doi.org/10.47604/ijf.1024.
[14] Walia A, Gupta AK, Sharma V. Role of bioactive compounds in human health. Acta Sci. Med. Sci. 2019 Sep;3(9):25-33. Role of bioactive compounds in human health - Google Scholar.
[15] Abdelmonem BH, Kamal LT, Elbaz RM, Khalifa M, Abdelnaser A. From contamination to detection: The growing threat of heavy metals. Heliyon. 2025 Jan 5. https://doi.org/10.1016/j.heliyon.2025.e41713.
[16] Smith DR, Nordberg M. General chemistry, sampling, analytical methods, and speciation. InHandbook on the Toxicology of Metals 2015 Jan 1 (pp. 15-44). Academic Press. https://doi.org/10.1016/B978-0-444-59453-2.00002-0.
[17] Cruz JC, de Souza ID, Lanças FM, Queiroz ME. Current advances and applications of online sample preparation techniques for miniaturized liquid chromatography systems. Journal of Chromatography A. 2022 Apr 12;1668:462925. https://doi.org/10.1016/j.chroma.2022.462925.
[18] Buzna c, horablaga mn, panda a, petru ra, luana sa, camelia ur, florin sa. Ash content in seeds of some industrial hemp genotypes. Life science and sustainable development. 2024 Dec 29;5(2):1-7. https://doi.org/10.58509/lssd.v5i2.295.
[19] Tarannum R, Alam A, Hasan MS, Rabby G, Raiyan A, Parvin R, Alam M, Zahid MA. Development of oats flour and bitter gourd fortified cookies: Effects on physicochemical, antioxidant, antimicrobial, and sensory attributes. PloS one. 2025 Jan 9;20(1):e0316575. https://doi.org/10.1371/journal.pone.0316575.
[20] Dogara AM. Biological Activity and Taxonomy of Persea americana Mill: A Systematic Review. EAJSE [Internet]. 2024 May 2 [cited 2025 May 22];10(2):46-71. Available from: https://eajse.tiu.edu.iq/index.php/eajse/article/view/19.
[21] Stefanović O, Radojević I, Vasić S, Čomić L. Antibacterial activity of naturally occurring compounds from selected plants. Antimicrobial Agents. 2012 Sep 12;1:25.
[22] Milenković K, Mrmošanin J, Mitov D, Petrović S, Tošić S, Mišić IR, Pavlović A. Evaluation of icp aes method for element determination in rosa corymbifera borkh. Samples. In3rd international symposium on biotechnology 2025 (p. 561). https://doi.org/10.46793/SBT30.69KM.
[23] Li H, Wang Q, Wang H, Li L, Wang X, Wu T, Wang C, Qian Y, Wang X, Xia Y, Xie J. LCLN-CA: A Survival Regression Analysis-Based Prediction Method for Catechin Content in Yunnan Sun-Dried Tea. Horticulturae. 2024 Dec 11;10(12):1321. https://doi.org/10.3390/horticulturae10121321.
[24] Massadeh AM, Alhusban AA. A developing method for preconcentration and determination of Pb, Cd, Al and As in different herbal pharmaceutical dosage forms using chelex-100. Chemical Papers. 2021 Jul;75:3563-73. https://doi.org/10.1007/s11696-021-01592-0.
[25] Mayer ML, Vyklicky Jr LA. The action of zinc on synaptic transmission and neuronal excitability in cultures of mouse hippocampus. The Journal of Physiology. 1989 Aug 1;415(1):351-65. https://doi.org/10.1113/jphysiol.1989.sp017725.
[26] Martínez-Ballesta MC, Dominguez-Perles R, Moreno DA, Muries B, Alcaraz-López C, Bastías E, García-Viguera C, Carvajal MJ. Minerals in plant food: effect of agricultural practices and role in human health. A review. Agronomy for sustainable development. 2010 Apr 1;30(2):295-309. https://doi.org/10.1111/j.1750-3841.2010.01606.x.
[27] Adamczyk-Szabela D, Markiewicz J, Wolf WM. Heavy metal uptake by herbs. IV. Influence of soil pH on the content of heavy metals in Valeriana officinalis L. Water, Air, & Soil Pollution. 2015 Apr;226:1-8. https://doi.org/10.1007/s11270-015-2360-3.
[28] Harris GK, Marshall MR. Ash analysis. Food analysis. 2017:287-97. https://doi.org/10.1007/978-3-319-45776-5_16.
[29] Ismail BP. Ash content determination. InNielsen's Food Analysis Laboratory Manual 2024 Jun 21 (pp. 129-131). Cham: Springer International Publishing. https://doi.org/10.1007/978-3-031-44970-3_14.
[30] Nielsen SS. Determination of moisture content. Food analysis laboratory manual. 2010:17-27. https://doi.org/10.1007/978-1-4419-1463-7_3.
[31] Mauer LJ. Moisture and total solids analysis. InNielsen's Food Analysis 2024 Jun 25 (pp. 233-260). Cham: Springer International Publishing. https://doi.org/10.1007/978-3-031-50643-7_15.
[32] Churkova B. Content of crude protein, crude fiber and crude ash in dry mass of birdsfoot trefoil varieties and populations. American Journal of Agricultural Science and Technology. 2013;1(3):77-83. https://doi.org/10.7726/ajast.2013.1007.
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The author has made the research data available. If you have any questions about accessing the data, please contact me at [chnar.mahmud@su.edu.krd].
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