Authors: Karam Ahmed Thiab1 & Mohamed Salim2
1Conservative Department, Faculty of Dentistry, Tishk International University, Erbil, Iraq
2Conservative Department, Faculty of Dentistry, Tishk International University, Erbil, Iraq
Abstract: Aim of study: This study aims at comparing the anti-microbial efficacy of irrigation with Biopure MTAD against irrigation with 5.25 % NaOCl, CHX, NaOCl/MTAD, and CHX/MTAD in E. faecalis-root canals. Materials and Methods: Thirty (30) human single-rooted lower premolar teeth were extracted and de-coronated at the cemento-enamel junction (CEJ). Then, the roots were instrumented by k-file until the size is 40. Furthermore, these teeth were exposed to bacterial contamination in brain heart infusion (BHI) for 48 h. The contaminated samples were divided into 5 groups (MTAD, NaOCl, CHX, NaOCl / MTAD, and CHX / MTAD). The dentin chips in the canals of the samples were removed using a sterile low-speed handpiece round bur and transferred to BHI. The media was cultured, and the bacterial colonies were counted and recorded as CFU/mL. Results: NaOCl / MTAD showed a significantly higher antimicrobial activity compared to 5.25 % NaOCl, MTAD, CHX, and CHX / MTAD. Conclusion: The antimicrobial activity of a combined NaOCl and Biopure MTAD was strongest against E. faecalis compared to the other irrigant solutions.
Keywords: NaOCl, CHX, E. faecalis
Published: January 19, 2023
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