Eurasian Journal of Science and Engineering
Volume 8 Issue 3 Article 25
Detection of Some Virulence Factors in Staphylococcus Aureus Isolated from Different Sources in Erbil City
Authors: Berivan Ali Ahmad Hasary 1 & Payman Ali Kareem2
1Food Technology Department, College of Agriculture Engineering Science, Salahaddin University, Erbil, Iraq
2Food Technology Department, College of Agriculture Engineering Science, Salahaddin University, Erbil, Iraq
Abstract: A total of 110 different samples were collected randomly from November 2021 to the end of February 2022 in Erbil city, in order to isolate different types of Staphylococcus, which are isolated from different sources like (milk, cheese, ice cream, meat, udder, hand worker, and nose worker). Staphylococcus aureus was examined for antibiotic susceptibility. The isolates were determined by the disk diffusion method. Twelve types of antibiotics have been chosen. S. aureus is more resistant to the Ciprofloxacin antibiotic and less resistant to the Streptomycin antibiotic. The virulence factors that the results have shown the ability of S. aureus to produce hemolycin in 59.7%, DNase in 17.7%, urease in 33.9%, lecithanase in 41.9%, lipase in 30.6%, B-Lactamease in 43.5%, coagulase enzyme in 61.3% and also protase in 61.3%. The biofilm forming ability of the isolates was investigated by the microtiter plate method. The production of biofilms by S. aureus isolates is 100%. 39.7% of the biofilm-positive S. aureus strains were strong producers, 35.5% were moderate producers, 17% were weak producers, and 7.5% were none.
Keywords: Staphylococcus Aureus, Virulence Factor, Antibiotics, Biofilm, Microtiter Plate Method
Published: January 5, 2023
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