Eurasian Journal of Science and Engineering
Volume 8 Issue 3 Article 23
In-Vitro Antioxidant Activity of Avocado Fruit Oil in Erbil, Kurdistan Region
Authors: Amani Tahsin1 & Rojan Arif2 & Esra Bayrakdar3 & Subasini Uthirapathy4
1Department of Pharmaceuticals Basic Sciences, Faculty of Pharmacy, Tishk International University, Erbil, Kurdistan Region, Iraq
2Department of Pharmaceuticals, Faculty of Pharmacy, Tishk International University, Erbil, Kurdistan Region, Iraq
3Department of Pharmaceuticals, Faculty of Pharmacy, Tishk International University, Erbil, Kurdistan Region, Iraq
4Department of Pharmacology, Faculty of Pharmacy, Tishk International University, Erbil, Kurdistan Region
Abstract: Many human diseases are linked to the accumulation of free radicals. Antioxidants can scavenge free radicals, lessening their impact. As a result, searching for naturally occurring antioxidants in plants is critical. This research aimed to investigate avocado’s antioxidant and free radical scavenging properties (Persea Americana Mill). Several standard methods for determining antioxidant and free radical scavenging activity were applied, including a spectrophotometer. Avocado fruit oil (AFO) has the highest overall antioxidant capacity, followed by Avocado seed oil (ASO). Based on ABTS and hydroxyl radical scavenging activity, IC50 values of 62.99 ug/ml and 50.68 ug/ml, respectively. According to these results, the AFO had a more active radical scavenging system than the ASO. AFO showed the strongest inhibitory activity in the lipid peroxidation inhibition experiment, with an IC50 of 318.8 ug/ml; however, ASO’s lipid peroxidation is 315.2 ug/ml, which is extremely similar to the IC50 of value for AFO. The nitric oxide radical scavenging activity and reduced glutathione assays were also in the AFO>ASO order. A positive correlation (p<0.001) was found between free radical scavenging efficiency and lipid peroxidation inhibition activities. Avocado fruit oil contains natural antioxidants and can serve as a free radical scavenger or inhibitor. As a result, Avocado may be a beneficial oil-based treatment for various illnesses caused by free radicals.
Keywords: Persea Americana, Free Radicals, ABTS, Antioxidant, Glutathione, ABTS
Published: January 5, 2023
References
A.O.A.C. (2000). Official Methods of Analysis 17th Edition, Association of Official Agric. Chem. Washington D.C. 1970.
Arancibia, C.; Riquelme, N.; Zúñiga, R.; Matiacevicha, S. (2017). Comparing the effectiveness of natural and synthetic emulsifiers on oxidative and physical stability of avocado oil-based nanoemulsions. Innov. Food Sci. Emerg. Technol. 44, 159–166.
Bora, P.S.; Narain, N.; Rocha, R.V.; Paulo, M.Q. (2001). Characterization of the oils from the pulp and seeds of avocado (cultivar: Fuerte) fruits. Grasas Aceites, 52, 171–174.
Buenrostro, M., López-Munguia, A.C (1986). Enzymatic extraction of avocado oil. Biotechnol. Lett, 8, 505–506.
Costagli, G.; Betti, M (2015.) Avocado oil extraction processes: Method for cold-pressed high-quality edible oil production versus traditional production. J. Agric, 46, 115–122.
Dos Santos, M.; Alicieo, T.V.; Pereira, C.M.; Ramis-Ramos, G.; Mendonça, C.R (2014). Profile of bioactive compounds in avocado pulp oil: Influence of the drying processes and extraction methods. J. Am. Oil Chem, Soc. 91, 19–27.
Dubois, V.; Breton, S.; Linder, M.; Fanni, J.; Parmentier, M (2007). Fatty acid profiles of 80 vegetable oils with regard to their nutritional potential. Eur. J. Lipid Sci. Technol, 109, 710–732.
Flores M, Saravia C, Vergara CE, Avila F, Valdes H, Ortiz-Viedma J. (2019). Avocado oil: Characteristics, properties, and applications. Molecules, 24 (11), 2172.
Flores, M.A.; Perez-Camino, M.C.; Troca, J (2014). Preliminary Studies on Composition, Quality and Oxidative Stability of Commercial Avocado Oil Produced in Chile. J. Food Sci. Eng, 4, 21–26.
Forero-Doria, O.; Flores, M.; Vergara, C.E.; Guzman, L (2017). Thermal analysis and antioxidant activity of oil extracted from pulp of ripe avocados. J. Therm. Anal. Calorim,130, 959–966.
Indian Pharmacopoeia, (1996): A – O, reference spectra & appendices, Volume 1 4th Edition Ministry of Health and Family Welfare, publisher Controller of Publications.
Indriyani, L.; Rohman, A.; Riyanto, S (2016). Physico-chemical characterization of Avocado (Persea americana Mill.) oil from three Indonesian avocado cultivars. Res. J. Med. Plants, 10, 67–78.
Ahamad, J., and Uthirapathy, S. (2021). Chemical Characterization and Antidiabetic Activity of Essential Oils from Pelargonium graveolens Leaves. ARO journal, https://dx.doi.org/10.14500/aro.10791
Khandelwal KR (2003). Practical Pharmacognosy Techniques and Experiments. Nirali Prakashan; Pune.
Kokate CK (2005). Practical Pharmacognosy. Vallabh prakashan; Delhi, 107-111.
Kosińska A, Karamać M, Estrella I, Hernández T, Bartolomé BA, Dykes GA. (2012). Phenolic compound profiles and antioxidant capacity of Persea americana Mill. Peels and seeds of two varieties. J Agric Food Chem, 60(18), 4613–4619.
Lunn J, Theobald H. (2006). The health effects of dietary unsaturated fatty acids. Nutr Bull, 31(3), 178–224.
Martinez-Nieto, L.; Moreno-Romero, M.V (1994). Sterolic composition of the unsaponifiable fraction of oil of avocado of several varieties. Grasas Aceites, 45, 402–403.
Martínez-Padilla, L.P.; Franke, L.; Xu, X.Q.; Juliano, P (2018). Improved extraction of avocado oil by application of sono-physical processes. Ultrason. Sonochemistry, 40, 720–726.
Ohkawa H, Ohishi N and Yagi K (2002). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction, Anal Biochem, J Med Food, 5, 69 – 78.
Ojewole J, Kamadyaapa DR, Gondwe MM, Moodley K, Musabayane CT. (2007). Cardiovascular effects of Persea americana Mill (Lauraceae) (avocado) aqueous leaf extract in experimental animals. Cardiovasc J Afr, 18(2), 69.
Govindharajan R, S Rastogi, M Vijayakumar, A K S Rawat, A Shirwaikar, S Mehrotra and P Pushpangadan (2003). Studies on antioxidant activities of Desmodium gangeticum. Biol Pharm Bull, 26, 1424 – 1427.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang Mr ICE, Evans C (1999). Antioxidant activity applying an improved ABTS radical cation decolorisation assay. Free radical Biol Med, 26, 1231.
Sales-Campos H, Reis de Souza P, Crema Peghini B, Santana da Silva J, Ribeiro Cardoso C. (2013). An overview of the modulatory effects of oleic acid in health and disease. Mini-Rev Med Chem, 13(2), 201–210.
Santana, I.; dos Reis, L.; Torres, A.; Cabral, L.; Freitas, S (2015). Avocado (Persea americana Mill.) oil produced by microwave drying and expeller pressing exhibits low acidity and high oxidative stability. Eur. J. Lipid Sci. Technol, 117, 999–1007.
Uthirapathy S. (2021). Cardioprotection effects of diosgenin from Dioscorea bulbifera against isoproterenol-induced myocardial infarction. Drugs and Cell Therapies in Hematology, 10(1), 887–896.
Thenmozhi S, Tarafaud Tahir and Subasini Uthirapathy, (2021). Pharmacognostical and Phytochemical Analysis of Stems of Vitex pinnata Linn. Research Journal of Phytochemistry, 15, 41-50. DOI:10.3923/rjphyto.2021.41.50 https://scialert.net/abstract/?doi=rjphyto.2021.41.50
Uthirapathy, S. (2021). Analgesic and Anti-inflammatory Activity of Withania somnifera Root Extract. Journal of Pharmaceutical Research International, 33(41A), 75-84. https://doi.org/10.9734/jpri/2021/v33i41A32304
Wang T, Hicks KB, Moreau R. (2002). Antioxidant activity of phytosterols, oryzanol, and other phytosterol conjugates. J Am Oil Chem Soc, 79 (12), 1201–1206.
Wang W, Bostic TR, Gu L. (2010). Antioxidant capacities, procyanidins and pigments in avocados of different strains and cultivars. Food Chem, 122(4), 1193–1198.
Tripathi Y. B, and M. Sharma (1998). Comparison of the antioxidant action of the alcoholic extract of Rubia cordifolia with Rubiadin. Indian J Biochem Biophy. 35, 313 – 316.
Zhang Z, Huber DJ, Rao J. (2013). Antioxidant systems of ripening avocado (Persea americana Mill.) fruit following treatment at the preclimacteric stage with aqueous 1-methylcyclopropene. Postharvest Biol Technol, 76, 58–64.