Eurasian Journal of Science and Engineering
Volume 8 Issue 3 Article 16
Griess Assay of Salivary Nitrite Content with Extraction Preconcentration
Authors: Harez Sdiq Faraj1 & Nabil Adil Fakhre2
1Department of Chemistry, College of Education, Salahaddin University-Erbil, Erbil, Iraq
2Department of Chemistry, College of Education, Salahaddin University-Erbil, Erbil, Iraq
Abstract: Nitrite has been determined using a simple and sensitive spectrophotometric method. The suggested procedure is based on the diazotization reaction of p-bromoaniline in the presence of nitrite ion in a hydrochloric acid media, which produces diazonium salt. This salt is then combined with paracetamol in an ammonium hydroxide medium to produce an orange azo color with a maximum wavelength (λ max.) of 460 nm, molar absorptivity (ε) is 3.8432 x 103 L. mol-l.cm-1, and Beer’s law was established between 0.3-12.0 µg. mL-1. It displays LOD, LOQ, and R2 values of 0.2 µg. mL-1, 0.3 µg. mL-1, and 0.9994 respectively. The dye can be extracted into chloroform. The yellow chloroform extract displays a maximum wavelength (λ max.) of 410 nm, molar absorptivity (ε) is 9.93 x103 L. mol-l.cm-1, and Beer’s law was observed between 0.1-5.0 µg. mL-1. The LOD, LOQ, and R2 values for the suggested extraction procedure were 0.05 µg. mL-1, 0.1 µg. mL-1, and 0.9996, respectively. The suggested method was successfully applied on samples of a human saliva.
Keywords: Nitrite, Griess Assay, Spectrophotometry, Liquid-Liquid Extraction, Paracetamol
Published: January 3, 2023
References
Adelana, S. M. A. (2005). Nitrate health effects. Water encyclopedia, 4, 30-42.
Brizzolari, A., Dei Cas, M., Cialoni, D., Marroni, A., Morano, C., Samaja, M., Paroni, R., & Rubino, F. M. (2021). High-Throughput Griess Assay of Nitrite and Nitrate in Plasma and Red Blood Cells for Human Physiology Studies under Extreme Conditions. Molecules, 26(15), 4569.
Caroleo, F., Diedenhofen, G., Catini, A., Di Natale, C., Paolesse, R., & Lvova, L. (2022). Phosphorous (V) Corrole Fluorophores for Nitrite Assessment in Environmental and Biological Samples. Chemosensors, 10(3), 107.
Coviello, D., Pascale, R., Ciriello, R., Salvi, A. M., Guerrieri, A., Contursi, M., Scrano, L., Bufo, S. A., Cataldi, T. R., & Bianco, G. (2020). Validation of an analytical method for nitrite and nitrate determination in meat foods for infants by ion chromatography with conductivity detection. Foods, 9(9), 1238.
Diaconu, R., Vasilov, M., & Mancaş, G. (2001). Colorimetric assay of salivary nitrite content. synthesis, 6, 9.
Garcia Torres, E., Perez Morales, R., Gonzalez Zamora, A., Rios Sanchez, E., Olivas Calderon, E. H., Alba Romero,
J. d. J., & Calleros Rincon, E. Y. (2022). Consumption of water contaminated by nitrate and its deleterious effects on the human thyroid gland: a review and update. International Journal of Environmental Health Research, 32(5), 984-1001.
Hamoudi, T. A., Jalal, A. F., & Fakhre, N. A. (2020). Determination of nitrite in meat by azo dye formation. Sys Rev Pharm, 11(6), 535-542.
Kokhasmile, D. M. (2016). Spectrophotometric Determination of Metronidazole in some Pharmaceutical Products and Determination of Nitrite and Nitrate in Water and Soil Samples Using Diazo-azatization Coupling Reaction, MSc. Thisis, University of salahaddin-Erbil,15,21,36.
Li, R., Yu, J. C., Jiang, Z., Zhou, R., & Liu, H. (2003). A solid-phase fluorescent quenching method for the determination of trace amounts of nitrite in foods with neutral red. Journal of Food and Drug Analysis, 11(3), 251-257.
Mikuška, P., & Večeřa, Z. (2003). Simultaneous determination of nitrite and nitrate in water by chemiluminescent flow-injection analysis. Analytica Chimica Acta, 495(1-2), 225-232.
Mohammad, H. F., & Fakhre, N. A. (2021). Liquid-Liquid Extraction of Copper (II) in a Synthetic Sample using a New Macrocyclic Compound. Rafidain Journal of Science, 30(4), 11-21.
Moshoeshoe, M. N., & Obuseng, V. (2018). Simultaneous determination of nitrate, nitrite and phosphate in environmental samples by high performance liquid chromatography with UV detection. South African Journal of Chemistry, 71, 79-85.
Mousavi, Z. H., & Shirkhanloo, H. (2009). Spectrophotometric determination of nitrite based on its catalytic effect on the reaction of nuclear fast red and potassium bromate. Journal of the Serbian Chemical Society, 74(8-9), 985-992.
Mzban, Q., Bahjat, S., & Hassan, M. J. M. (2020). DISPERSIVE LIQUID-LIQUID MICROEXTRACTION AND SPECTROPHOTOMETRIC DETERMINATION OF CEFAZONE AND PHENYLEPHRINE HYDROCHLORIDE IN THEIR PURE FORMS AND PHARMACEUTICAL PREPARATIONS. Plant Archives, 20(2), 6771-6777.
Numan, A., Al-Nedhary, A., Al-Hamadi, M., Saleh, S., Ghaleb, F., & Galil, M. (2021). Novel Spectrophotometric Method with Enhanced Sensitivity for the Determination of Nitrite in Vegetables. JJEES, 12(1), 13-21.
Orabi, A. (2013). Determination of uranium after separation using solvent extraction from slightly nitric acid solution and spectrophotometric detection. Journal of radiation research and applied sciences, 6(2), 1-10.
Parvizishad, M., Dalvand, A., Mahvi, A. H., & Goodarzi, F. (2017). A review of adverse effects and benefits of nitrate and nitrite in drinking water and food on human health. Health Scope, 6(3).
Rahim, S., Fakhri, N., & Bashir, W. (1983). Indole as a reagent for nitrite in aqueous solution. Microchemical Journal, 28(4), 479-484.
Rasheed, C. M. (2003). Determination of Nitrite and Nitrate in Water and Soil Samples Using Bach and Flow-Injection Spectrophotometric Methods. MSc Thesis University of Salahaddin-Erbil
6-8,49-50,58,61,105,106.
Rished, C. M., & Fakhre, N. A. (2008). Spectrophotometric determination of nitrite in curing meat samples basing on the nitrosation reaction. Wasit Journal for Science & Medicine, 1, 23-30.
Roohparvar, R., Shamspur, T., Mostafavi, A., & Bagheri, H. (2018). Indirect ultra-trace determination of nitrate and nitrite in food samples by in-syringe liquid microextraction and electrothermal atomic absorption spectrometry. Microchemical Journal, 142, 135-139.
Schrenk, D., Bignami, M., Bodin, L., Chipman, J. K., del Mazo, J., Grasl-Kraupp, B., Hogstrand, C., Hoogenboom, L. R., Leblanc, J.-C., & Nebbia, C. S. (2020). SCIENTIFIC OPINION. assessment, 31, 32.
Senra-Ferreiro, S., Pena-Pereira, F., Lavilla, I., & Bendicho, C. (2010). Griess micro-assay for the determination of nitrite by combining fibre optics-based cuvetteless UV–Vis micro-spectrophotometry with liquid-phase microextraction. Analytica Chimica Acta, 668(2), 195-200.
Tarafder, P., & Roychowdhaury, S. (2018). A novel method for the extraction spectrophotometric determination of nitrate and nitrite in water, waste water and effluent. Int J Anal Bioanal Tech, 1, 1-6.
Tatarczak-Michalewska, M., Flieger, J., Kawka, J., Płaziński, W., Flieger, W., Blicharska, E., & Majerek, D. (2019). HPLC-DAD determination of nitrite and nitrate in human saliva utilizing a phosphatidylcholine column. Molecules, 24(9), 1754.
Veena, K., & Narayana, B. (2009). Spectrophotometric determination of nitrite using new coupling agents.
Wang, Y., Wang, Y., Huang, C., Chen, T., & Wu, J. (2020). Ultra-Weak Chemiluminescence Enhanced by Cerium-Doped LaF3 Nanoparticles: A Potential Nitrite Analysis Method. Frontiers in Chemistry, 8, 639.
WHO. (2003). Nitrate and nitrite in drinking-water: Background document for development of WHO Guidelines for Drinking-water Quality.
Zhang, Y., Nie, J., Wei, H., Xu, H., Wang, Q., Cong, Y., Tao, J., Chu, L., Zhou, Y., & Wu, X. (2018). Electrochemical detection of nitrite ions using Ag/Cu/MWNT nanoclusters electrodeposited on a glassy carbon electrode. Sensors and Actuators B: Chemical, 258, 1107-1116.
Zhao, J., Wang, J., Yang, Y., & Lu, Y. (2015). The determination of nitrate and nitrite in human urine and blood by high-performance liquid chromatography and cloud-point extraction. Journal of chromatographic science, 53(7), 1169-1177.