Authors: Hemn Abdulwahab Ahmad1 & Karzan Abdulmuhsin Mohammad2
1Biology Department, College of Education, Salahaddin University, Erbil, Iraq
2General Directorate of Scientific Research Center, Salahaddin University, Erbil, Iraq
Abstract: The yeast Candida albicans is one of the most common pathogens of medical concern. It can cause diseases ranging from superficial and topical infections to systematic disseminated medical illnesses. Eradication of biofilms and its disruption is a great medical challenge to treat infections caused by Candida albicans. The yeast could develop antifungal resistant throughout decades, which is mainly due to their dimorphic phenomenon and biofilm construction. The use of medicinal plants has been used by ancient populations of humanity civilizations for the cure of fungi. Many chemical ingredients have been proved to have impact on the growth and biofilms of fungi. In this investigation, certain medical plants such as Galls of Quercus infectoria (Q. infectoria), Cyprus rotundus, and Alum extract against of Candida albicans atcc10231 in vitro and biofilm development. The well diffusion method was used to determine the sensitivity and Minimal Inhibitory Concentration (MIC). Zones of inhibition were recorded in concentration of 200% of the extracts. The ethanolic extract of Cyprus rotundus and interaction of and Alum showed best inhibitory zone of 23 mm. While the watery extract Q. infectoria showed an inhibition zone of 22 mm. The MIC records were 20000 µg/ml for Cyprus rotundus was, 1250 µg/ml for Q. infectoria, 15000 µg/ml for Alum, and 80000 for an interaction of Alum and Q. infectoria extracts respectively. Biofilm disruption were assessed. Extracts of Quercus gall, Cyprus rotundus, and alum extract were found to have a substantial inhibitory effect on Candida albicans ATCC10231 growth inhibition, as well as the production of biofilms. This study is the first approach to study the biofilm of Candida albicans in RPMI medium in the Kurdistan Region of Iraq. The combination of the used extracts can enlighten the synergetic impact of those extracts and other medications to be used for treatment of fungal infections.
Keywords: Antifungal Activity, Q. infectoria, Cyprus Rotundus, Alum Extract, Candida Albicans ATCC10231, Well Diffusion Method, Disrupt
Published: June 16, 2022
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