Authors: Fatiha El Babili1 & Nicole Linda-Mweze2 & Caroline Vincent 2 & Romain Laleman3 & Arthur Hourugou3
1Jardin Botanique Henri Gaussen Université Paul Sabatier Toulouse III, Toulouse, France
2Ecole préparatoire de Chimie, Lycée d’Enseignement Général, 26, Toulouse, France
3IUT Paul Sabatier Toulouse III – site Castres Avenue Georges Pompidou F- 81104 Castre, France
Abstract: Background and Aim: As part of our antioxidant plants screening project, we conducted a phytochemical study on their antioxidant activity to provide a standard and reliable assessment tool. To carry out this extensive screening, in compliance with regulatory requirements in pharmaceutical field, we have undertaken validation of our DPPH assay. This validation is in accordance with ICH standards. Indeed, this validation procedure has a very broad scope. It applies to any analysis procedure for plant raw materials control.
Method: DPPH test created by Blois and adapted for our raw extracts study is carried out using a UV spectrophotometer at 516 nm, as a monitoring wavelength. The ICH standards are then studied using an appropriate extracts and samples number for relevant statistical analysis. The reference substance chosen is Trolox.
Results: To validate this method, we will quantify antioxidant properties in two plant drugs traditionally known for their property such as Argania spinosa (fruits and leaves) and Lawsonia inermis (ethanolic extracts and decoctions). Our results allow to specify and quantify these properties and to confirm traditional ancient use.
Conclusions: The method developed is therefore recommended as a quality control protocol in phytochemical screening. This last screening is always necessary before starting anticancer properties plants study. Plants are gradually regaining interest and a preliminary screening method will can be included in specific monograph. Thus these monographs could help to promote the heritage of traditional medicine; still alive and to which the WHO seeks to restore its place (latest resolution 2014-2023).
Keywords: ICH, Linearity, Antioxydant Ability, Argania spinosa, Lawsonia inermis, Trolox
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