Antimicrobial Activity of Cyperus rotundus Linn. Extracts and Phytochemical Screening

Authors :Khalid Karzan1 & Bushra Shnawa2 & Shorish Gorony3
1&2&3Soran University, ScienceFaculty, Biology Department, Erbil, Iraq

Abstract:  This study aimed to evaluate the antimicrobial activity of C. rotundus rhizomes and tuber together, leaves and oil against several bacteria and three species of fungi. The plant was collected from Degala, Erbil, Kurdistan, Iraq. Antimicrobial activity of Crude aqueous and ethanolic extracts was examined by inoculating bacteria and fungi on media containing plant extract, and agar well diffusion method. The study revealed the effect of plant by evaluating microbial growth and inhibition zone measurement. Aqueous extract of leave had ability to suppress growth of E. coli, and both aqueous and ethanolic extracts of rhizome-tuber figured out antibacterial activity against S. aureus and E. coli, positively. Moreover, all selected bacteria and fungi were susceptible to ethanolic extract of rhizome- tuber, with different inhibition zones. Cyperus oil had no potential inhibitory effect against bacteria and fungi, excluding C. albicans. By performing phytochemical screening for rhizome-tuber extracts eleven secondary metabolites were detected.

Keywords:  Cyperus Rotundus, Antimicrobial Activity, Aqueous Extract, Ethanolic Extract, Phytochemicals


doi: 10.23918/eajse.v3i2p82


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References
Aharoni, A., Jongsma, M. A., & Bouwmeester, H. J. (2005). Volatile science? Metabolic engineering
of terpenoids in plants. Trends in Plant Science, 10(12), 594-602.
Al-Massarani, S., Al-Enzi, F., Al-Tamimi, M., Al-Jomaiah, N., Al-amri, R., Başer, K. H. C., … &
Demirci, B. (2016). Composition & biological activity of Cyperus rotundus L. tuber
volatiles from Saudi Arabia. Natural Volatiles & Essential Oils, 3(2).
Bhattarai, N. K. (1993). Folk herbal remedies for diarrhoea and dysentery in central Nepal.
Bown, D. (1995). Encyclopaedia of Herbs and their Uses. London:Dorling Kindersley.

Dang, G. K., Parekar, R. R., Kamat, S. K., Scindia, A. M., & Rege, N. N. (2011). Antiinflammatory
activity of Phyllanthus emblica, Plumbago zeylanica and Cyperus rotundus in acute models
of inflammation. Phytotherapy Research, 25(6), 904-908.
Dugger, H. (2002). U.S. Patent Application No. 10/230,075.
Harbone, B. (1984). Phytochemical Methods. 2nd. New York, Champan, 4, 4-7.
Himaja, N., Anitha, K. Joshna, A., & Pooja, M. (2014). Review article on health benefits of Cyperus
rotundus. Indian Journal of Drugs, 2(4), 136-141.
Him-Che, Y. (1985). Handbook of Chinese herbs and formulas. Institute of Chinese Medicine, Los
Angeles, 1, S219-S224.
Kabbashi, A. S., Mohammed, S. E. A., Almagboul, A. Z., & Ahmed, I. F. (2015). Antimicrobial
activity and cytotoxicity of ethanolic extract of Cyperus rotundus L. American Journal of
Pharmacy and Pharmaceutical Sciences, 2(1), 1-13.
Kage, D. N., Seetharam, Y. N., & Malashetty, V. B. (2009). In vitro antibacterial property and
phytochemical profile of Trichosanthes cucumerina L. var. cucumerina. Advances in
Natural and Applied Sciences, 3(3), 438-442.
Kilani, S., Sghaier, M. B., Limem, I., Bouhlel, I., Boubaker, J., Bhouri, W., … & Ghedira, K. (2008).
In vitro evaluation of antibacterial, antioxidant, cytotoxic and apoptotic activities of the
tubers infusion and extracts of Cyperus rotundus. Bioresource technology, 99(18), 9004-
9008.
Komai, K. & Ueki, K. (1975). Chemical properties and behavior of poly phenolic substances in
purple nutsedge (Cuperus rotundus L.). Weed Research, 20(2), 66-71.
Komai, K. Iwamura, J., & Ueki, K. (1997). Isolation, identification and physiological activities of
sesquiterpenes in purple nutsedge. Weed Research, 22,14-18.
Mutalib, L. Y. (2015). Comparative physicochemical, phytochemical and biological study of
botanically related species from Brassicaceae family grown in Kurdistan Region, Iraq.
Asian Journal of Research in Pharmaceutical Science, 5(3), 168-174.
Naqishbandi, A. (2014). Plants used in Iraqi traditional medicine in Erbil-Kurdistan region. Zanco
Journal Medical Science, 18(3), 811-815.
Narwal, S. S., Pahuja, S. S., & Gupta, K. (1992). Allelopathic effects of stubble extract of pearl
millet on seed germination and seedling growth of fodder crops’. In Proceedings of the
First National Symposium on Allelopathy in Agro ecosystems, Tauro, P. and SS Narwal
(Eds.). Indian Society of Allelopathy, Haryana Agricultural University, Hisar, India (p.
21).
Nima, Z. A., Jabier, M. S., Wagi, R. I., and Hussain, H. A. (2008). Extraction, identification and
antibacterial activity of Cyperus oil from Iraqi C. Rotundus. Eng Technol, 26(10), 1156-
1159.
Parekh, J., & Chanda, S. (2006). In-vitro antimicrobial activities of extracts of Launaea procumbens
roxb.(Labiateae), Vitis vinifera l.(Vitaceae) and Cyperus rotundus l.(Cyperaceae. African
Journal of Biomedical Research, 9(2).
Peerzada, A. M., Ali, H. H., Naeem, M., Latif, A. H. B. & Tanveer, A. (2015). Cyperus rotundus L.:
Traditionaluses, phytochemistry and pharmacological activities. Journal of
Ethnopharmacology, 174, 540-560.
Prasad,M.P.(2014). Analysis of antimicrobial compounds in Cyperus rotundus andAzadirachta
indica against human pathogen. International.Journal of
Current.Microbiol.Applied.Science, 3(3), 206-210.
Rojas, R., Bustamante, B., Bauer, J., Fernández, I., Albán, J. & Lock, O. (2003). Antimicrobial
activity of selected Peruvian medicinal plants. Journal of ethnopharmacology, 88(2), 199-
204.
Santos, P. R. V. D., Oliveira, A. C. X. D. & Tomassini, T. C. B. (1995). Controle microbiológico de
produtos fitoterápicos. Rev. Farm. Bioquim. University of Säo Paulo, 31(1), 35-38.
Sharma, R., & Gupta, R. (2007). Cyperus rotundus extract inhibits acetylcholinesterase activity from
animal and plants as well as inhibits germination and seedling growth in wheat and tomato.
Life sciences, 80(24), 2389-2392

Sharma, S. K., & Singh, A. P. (2011). Antimicrobial investigations on rhizomes of Cyperus rotundus
Linn. Der Pharmacia Lettre., 3(3), 427-431.
Singh, N. B., & Singh, P. N. (1986). A new flavonol glycerol glycoside from the mature tubers of
Cyperus rotundus L. Journal of the Indian Chemical Society, 63(4), 450-450.
Singh, N. B., Pandey, B. N., & Singh, A. (2009). Allelopathic effects of Cyperus rotundus extract in
vitro and ex vitro on banana. Acta Physiologiae Plantarum, 31(3), 633-638.
Singh, P. N., & Singh, S. B. (1980). A new saponin from mature tubers of Cyperus rotundus.
Phytochemistry, 19(9), 2056.
Sivapalan, S.R. (2013). Medicinal uses and pharmacological activities of Cyperus rotundus Linn- A
review. International Journal of Scientific and Research Publication, 3(5), 1-8.
Sundaram, M. S., Sivakumar, T., & Balamurugan, G. (2008). Anti-inflammatory effect of Cyperus
rotundus Linn. Leaves on acute and subacute inflammation in experimental rat models.
Biomedicine, 28, 302-304.
Sydor, J. R., Normant, E., Pien, C. S., Porter, J. R., Ge, J., Grenier, L., and Patterson, J. (2006).
Development of 17-allylamino-17-demethoxygeldanamycin hydroquinone hydrochloride
(IPI-504), an anti-cancer agent directed against Hsp90. Proceedings of the National
Academy of Sciences, 103(46), 17408-17413.
Uddin, S. J., Mondal, K., Shilpi, J. A., & Rahnan,