Spectrophotometric Study and Analytical Applications of Iron (III)-Chelate System with (Mordant Blue-9) Reagent in Aqueous Solution

Author :Dheyaa JirjeesYaqoob1
1College of Agriculture, Salahaddin University, Erbil, Iraq

Abstract:  The reagent 6-(5-chloro-2-hydroxy-3-sulfophenylazo)-5-hydroxy-1-naphthalene sulfonic acid(Mordant blue – 9) is proposed as a spectrophotometric reagent for the determination of trace amounts ofiron (III). The proposed chromogenic reagent is sensitive and reacts with iron to form a stable brown 1:2 (Fe(III) – reagent) complex at pH 3.75, Kst = 9.824*109 L2
.mol-2.The apparent molar absorptivity of thiscomplex was 16000 l .mol -1 cm-1at 650 nm in aqueous solutions. Beer’s law is obeyed with the range of 6 – 75 µg /25 ml i.e 0.24 – 3 ppm. The reagent is relatively selective for iron III. The importance of this work arises from that it allows quantification and determination of Iron without initial expensive and fatigue separation or extraction procedure. Different analytical validations were determined (accuracy, precision, recovery, stability of the complex, study of interferences, etc.) to demonstrate the suitability of this work for the routinequality control labs. The aim of this work was to determine iron III in aqueous solution specrtophotometrically employing mordant blue-9 reagent. The developed method was applied for the determination of Fe III in urine samples. The results agree with AAS with RSD (±0.74 – ±2.64).

Keywords: Social Iron (III), Spectrophotometry, Mordant blue -9, Analytical Applications

doi: 10.23918/eajse.v3i1sip72

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Abdel-Ghafar, H.H., etal, (2015). Spectrophotometric determination of Iron and Copper ions in industrial
waste water, cooling water and scale of power station. Journal of Desalination and Water
Treatment, 55 (1), 69 – 106.
Bailer, J. C., Emelens, H. J., Nyholm, R., & Trotman-Dickenson, A. F. (1973). Comprehensive Inorganic
Chemistry. Oxford: Pergamon Press Ltd.
Barakat, S.A. (2017). Back flow injection spectrophotometric determination of selected Fluro quinolones.
Current Analytical Chemistry, 13 (6).
De Jong, J., den Das, J., Bathmann, U., Stoll, M., Kattner, G., Nolting, R.F., de Baar, H. (1998). Dissolved
iron at subnanomolar levels in the Southern Ocean as determined by ship-board analysis. Anal.
Chim. Acta., 377, p113-117.
Elsuccary, S., & Salem, A. (2015). Novel flow injection analysis methods for the determination of total
Iron in blood serum and water. Talanta, 131, 108-115.
Faizullah, A.T., & Townshend, A. (1985). Application of reducing column for metal speciation by flow
injection analysis. Analytica Chimica Acta, 167, 225-231.
Ghodvinde, V.J. et al , (2017). New analytical technique for determination of trace amount of Fe (III) by
using UV- visible spectrophotometric method with photometric reagent. Journal of Applicable
Chemistry, 6 (1), 107 – 114.
Hargis, L. G. (1988). Analytical Chemistry: Principles and Techniques. New Jersey: Prentice-Hall, Inc.
Holzbecher, Z., Divis, L., Kral, M., Sucha, L., & Vlacil, F. (1976). Hand Book of Organic Reagents in
Inorganic Analysis. Chichester: Ellis Horwood Limited.
Hussain,F., & Saeed,M.Sultana,N. (2006). Spectrophotometric method for quantitative determination of
iron (III) from iron polymaltose complex. Pak.J.Pharm.Sci., 19 (4), 299-303.

Jafarian, D., Saghaie, L., Movahedi, N. (2008). A new spectrophotometric method for direct determination
of iron (III) in serum. DARU 16 (2), 76.
Martin, D., Mayes, P., & Granner, D. (1985). Harpers Review of Biochemistry. California: 20th. Edn. Lang
Medical Publications.
Perrin, D. D., & Dempsey, B. (1974). Buffers for pH and Metal Ion Control. London: Champman and Hall
Schnell, S., Ratering, S., Jansen, K.H. (1998). Simultaneous determination of iron (III), iron (II), and
manganese (II) in environmental samples by ion chromatography. Environ. Sci. Technol., 32,
Sohrin, Y., Iwamoto, S., Akiyama, S., Fujita, T., Kugii, T., Obata, H., Nakayama, E., Goda, S., Fujishima,
Y., Hasegawa, H., Ueda, K., Matsui, M. (1998). Determination of trace elements in seawater by
fluorinated metal alkoxide glass-immobilized 8-hydroxyquinoline concentration and highresolution inductively coupled plasma mass spectrometry detection. Anal. Chim. Acta., 363.
Suwansaksri, J., Sookarun, S., Wiwanitkit, V., Boonchalermvichian, C., Nuchprayoon, I. (2003).
Comparative study on serum iron determination by different methods. Lab. Hematol. 9 (4), 234-
Taies, J., & Jassam, N. (2013). Spectrophotometric determination of trace amounts of Fe (III), Mn (III)
and Co (III) ions using Tetra (4-Bromophenyl) Porphyrin as a Ligand in DMF Solvent.
International Journal of Science and Technology, 3(3), 201 – 208.
Wilkinson, G. (1987). Comprehensive Coordination Chemistry. Oxford: Pergamon Press, Ltd.