First Derivative Spectrophotometric Determination of Cholesterol and Triglyceride in Human Serum

Authors :Nabil A. Fakhre¹ & Dashty K. Ali^2
1Department of Chemistry, College of Education, Salahaddin University, Erbil, Iraq

Abstract:   Cholesterol is present in tissues and plasma lipoproteins either as free cholesterol or combined with a long chain fatty acid as cholesterol ester. Triglycerides are the simplest lipids constructed from fatty acids and glycerol. Derivative spectrophotometric techniques were used for determination of individual and simultaneous determination of cholesterol and triglyceride. First derivative spectrophotometric technique was used for determination of cholesterol and triglyceride in serum samples in the concentration ranges (10 – 100 μg/ml) for cholesterol with correlation coefficient 0.9983 and detection limit 2.9 μg/ml with good precision and accuracy basing upon measuring height at 218 nm depending upon the values of the relative standard deviation percentage (RSD %) and the relative error percentage (Error %) for ten replicate measurements of three different concentration and (15 – 150 μg/ml) for triglyceride with correlation coefficient 0.9972 and detection limit 5.5 μg/ml with good precision and accuracy basing upon measuring peak – to – baseline at 242 nm depending upon the values of the relative standard deviation percentage (RSD %) and the relative error percentage (Error %) for ten replicate measurements of three different concentration.

Keywords:  Derivative, Determination, Cholesterol and Triglyceride

doi: 10.23918/eajse.v3i1sip137

Download the PDF Document from here.

References
David, P. (1971). An Introduction to Practical Biochemistry. London, UK. McGraw-Hill
Company Limited.
David, P. (1981). Principles of Biological Chemistry. Boston, USA: 2nd Edn. Willard Grant Press.
Frederick, A. B., William, H. B., & Jerry, M. (2004). Introduction to General, Organic and
Biochemistry. Belmont, CA: 7th Edn. Brooks/Cole-Thomson Learing Inc.
Gajera, H. P., Patel, S. V., & Golakiya, B. A. (2008). Fundamentals of
Biochemistry: a Text Book. UP, India: 1st Edn. IBDC.
Jen, W., Nora, G., Taiyi, K., & Charles, A. H. (2012). Regulation of triglyceride metabolism by
glucocorticoid receptor. Cell & Bioscience, 2(19), 1.
Osman, H., & Yap, K. C. (2006). Comparative sensitivies of cholesterol analysis using GC, HPLC
and spectrophotometric methods. The Malaysian Journal of Analytical Sciences, 2(10),
205-206.
Mariona, J., Jose, C. S., Maria, J. B., Anna, C., Neus, A., Jordi, R., Jose, R. M., Reinald, P., &
Manuel, P. (2010). When cholesterol is not cholesterol a note on the enzymatic
determination of its concentration in model systems containing vegetable extracts. Lipids in
Health and Disease, 9(65), 1.
Pundir, C. S., Bharvi, S. S., & Jagriti, N. (2010). Construction of an amperometric triglyceride
biosensor using PVA membrane bound enzymes. Clinical Biochemistry, 43, 467.
Robert, K. M., Dary, K. G., Peter, A. M., & Victor, W. R. (1993). Harpers Biochemistry. New York,
USA: 23rd Edn. Lange Medical Publication.
Russell, G. W., Mary, M. K., Parvin, P. W., Elizabeth, T. L., & Gary, L. M. (2008). Standardization
of measurements for cholesterol, triglycerides and major lipoproteins. Labmedicine, 39(8),
481.