Incidence and Molecular Identification of Escherichia Coli Harbouring Gentamicin Resistant Gene among Pregnant Women

Authors: Hassan M. Tawfeeq¹ & Ahmed M. Tofiq² & Kameran M. Ali^3
1&3Nursing Department, Kalar Technical Institute, Sulaimani Polytechnic University, Iraq
²Department of Biology, College of Education, University of Garmian, Kalar, Iraq

Abstract:   Pregnant-origin Gram negative enteric bacteria, particularly, Escherichia coli isolates were exposed to tests for resistance phenotype using Kirby-Bauer’s disk-diffusion assay and for gentamicin resistance genotype using aacC2gene, codifying for one specific aminoglycoside-modifying enzyme. The incidence of Enterobacteriaceae was 31 (21.8%), emerged from142 midstream urine (MSU) samples, including symptomatic (17.6%) and asymptomatic (25.7%) bacteriuria. Women with age brackets from 15-44 years were studied. Age group 25-29 years scored the highest incidence rate of infection (29.2%), while age group 35-39 years exhibited the lowest incidence rate. Data described previously were statistically insignificant (P value > 0.05). Remarkably, significant differences (P value <0.05) observed concerning gestational ages when third (32.81%) and second (14.81%) trimesters were noticed with the highest rate of bacteriuria, respectively. E. coli distinguished as the most predominant (41.9%), followed by Klebssiella pneumoniae (19.4%) and Pseudomonas aeruginosa (12.9%), consecutively. Seven other uropathogens were identified with a lesser frequency. Isolates subjected to numerous antibiotics and results revealed fully resistance to pencillin G and amoxicillin-clavulanic acid, while (96.8%) possessed resistant to amoxicillin, cephlexin, and vancomycin. However, amikacin still acting perfectly (90.3%) against all the isolates, especially towards E. coli (92.3%). Concurrently, the antimicrobial potency of streptomycin, ciprofloxacin, and ceftriaxone were (92.3%), (84.6%), and (77%) respectively, when assayed opposite E. coli strains solely. Phenotypically, (30.8%) of E. coli strains, which stand for about (32.3%) of the whole isolates, were resistance to gentamicin. 75% of phenotypically gentamicin resistance E. coli demonstrated genotypically as harbouring the aacC2 gene.

Keywords:  E. Coli, UTI, Pregnancy, Aacc2 Gene, Antibiogram

doi: 10.23918/eajse.v3i1sip117

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